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Henan Tongwei Medical Equipment Co., LtdCall us : +86 − −19139704654 // Email us : [email protected]
Activity-based protein profiling (ABPP) has emerged as a powerful chemical proteomic strategy to characterize enzyme function directly in native biological systems on a global scale Here we review the basic technology of ABPP the enzyme classes addressable by this method and the biological discoveries attributable to its application Key Words affinity label mass spectrometry proteomics
Genomic profiling of circulating tumor DNA derived from cell-free DNA (cfDNA) in blood can provide a noninvasive method for detecting genomic biomarkers to guide clinical decision making for cancer patients We developed a hybrid capture–based next-generation sequencing assay for genomic profiling of circulating tumor DNA from blood (FoundationACT)
Isolation culture and genomic characterization of human adenoma organoids We have developed an ongoing repository of organoids from patient-derived adenomas (n=17 including two high-risk sessile serrated adenomas) adenocarcinomas (n=4 including one colitis-associated cancer) and normal colon (n=9) All have been cryopreserved at early passage reestablished from frozen stock validated as
Hyaluronidase enzyme (HAase) has a role in the dissolution or disintegration of hyaluronic acid (HA) and in maintaining the heathy state of skin Bioassay-guided fractionation of Ravenala madagascariensis (Sonn ) organ extracts (leaf flower stem and root) testing for hyaluronidase inhibition was performed followed by metabolic profiling using LCndash S
Confocal microscopy of human gastric organoids shows that—similar to spheroids—they consist of a single layer of cylindrical highly polarised epithelial cells as indicated by E-cadherin and β-catenin immunolabelling (figure 3D upper panel) In contrast to the perfectly rounded spheroids the organoids exhibit folded gland-like structures typically found in healthy gastric mucosa
Probe-Seq allowed the isolation and profiling of RNA from fresh mouse frozen human and fresh chick retinas as well as gut cells from Drosophila melanogaster Aside from the different dissociation protocols Probe-Seq does not require species- or tissue-specific alterations To profile multiple cellular subtypes multiplexed Probe-Seq allows for iterative labeling sorting and re-labeling
Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology Traditional isolation methods for peroxisomes can take more than 1 h to complete and can be laborious to implement To address this we have now extended our prior work on rapid organellar isolation to peroxisomes via the development of a peroxisomally localized 3XHA epitope tag
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR Mestdagh P Lefever S Volders PJ Derveaux S Hellemans J Vandesompele J Sci Data 2016 Jul 3: 160052 Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression
In addition primary cells for example from human (blood) or from rodent (CNS) are available upon request for assay development Secondary screening for pharmacological evaluation of compound characteristics is part of our Integrated Drug Discovery expertise Mode of action biomarkers target engagement studies are provided for hit characterization and for lead finding and optimization
Infection and RNA isolation Human alveolar macrophages were plated into six well culture plates at 1 10 6 cells/ml in RPMI with 2% FCS containing 50 μg/ml of gentamicin and following overnight attachment were stimulated with B anthracis spores (1 MOI) for 6 h This time was chosen based on previous experiments showing that peak cytokine mRNA induction by spores occurred between 5 and
Antibody profiling of SCI plasma samples using SAS A high-quality hSC cDNA phage display library was generated from human spinal cord tissue of 18 Caucasians Human spinal cord cDNA fragments were cloned into the pVI phage display vectors in three reading frames resulting in a total library size of 2 44 10 6 independent clones The spinal
Specialized immune cell subsets are involved in autoimmune disease cancer immunity and infectious disease through a diverse range of functions mediated by overlapping pathways and signals However subset-specific responses may not be detectable in analyses of whole blood samples and no efficient approach for profiling cell subsets at high throughput from small samples is available
qRT-PCR receptome profiling is accurate precise and more sensitive than oligonucleotide microarrays We defined a signaling "receptome" that includes all human receptor serine-threonine and tyrosine kinases all cytokine and chemokine receptors as well as all receptors of the Toll-like Frizzled Notch and Patched families (Fig 1A and file S1)
Reference Profiles of ExRNAs in Normal Human Pregnancy Laurent Louise Chang University of California San Diego La Jolla CA United States Search 32 grants from Louise Laurent Search grants from University of California San Diego Share this grant:
We analyzed DNA from a panel of HTLV-1-infected cell lines and noninfected T-cell lines using a commercial STR kit and then analyzed the same DNA for individual STR markers followed by nondenaturing polyacrylamide gel electrophoresis This simplified method should facilitate routine confirmation of cell line identity in diverse laboratory settings Key words HTLV-1 ATLL STR profiling
Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein immunology view on bioRxiv By Seth J Zost Pavlo Gilchuk Rita E Chen James Brett Case Joseph X Reidy Andrew Trivette Rachel S Nargi Rachel E Sutton Naveenchandra Suryadevara Elaine C Chen Elad Binshtein Swathi Shrihari Mario Ostrowski Helen Y Chu
Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein This study describes the methodology used to efficiently generate a large library of highly-functional monoclonal antibodies directed against the SARS-CoV-2 spike (S) protein using the Single Cell Immune Profiling Solution
The transcriptional regulatory structure of plant genomes remains poorly defined relative to animals It is unclear how many cis -regulatory elements exist where these elements lie relative to promoters and how these features are conserved across plant species We employed the assay for transposase-accessible chromatin (ATAC-seq) in four plant species ( Arabidopsis thaliana Medicago
The direct comparison with previously described isolation protocols 14 22 23 revealed significantly higher recovery of viable cells by Liberase TL treatment (K M unpublished data) Compared to collagenase which is frequently used for the isolation of immune cells from the brain Liberase TL contains negligible levels of endotoxin
Agilent Technologies has added the SureSelect Human All Exon V6 V6+UTR and V6+COSMIC to its line of target-enrichment products The new products are designed to address the limitations of exome sequencing targeting hard-to-capture and multi-mapping regions The products also increase the sensitivity of variant calling and minimize false negative calls Agilent said
We next assembled a diverse panel of human prostate cancer cell lines to further characterize cell-surface antigens in the PrAd and NEPC subtypes The panel included established lines including CWR22Rv1 LNCaP NE1 3 DU145 NCI-H660 and LASCPC-01 as well as two developed cell lines named NB120914 and MSKCC EF1 NB120914 was initiated from
Cell preparation for droplet-based single-cell transcriptional profiling Schematic of experimental workflow Cultured human (HEK) and mouse (3T3) cells were dissociated mixed and further processed to analyse the transcriptomes of either live or fixed cells by Drop-seq Washed cells were gently resuspended in 2 volumes of ice-cold PBS then fixed by adding 8 volumes of ice-cold methanol
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Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt () analysis [version 2 peer review: 2 approved] Erin K Hanson Jack Ballantyne1 2 National Center for Forensic Science Orlando FL 32816-2367 USA Department of Chemistry University of Central Florida Orlando FL 32816-2366 USA Abstract Positive identification of the nature of
Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein immunology more details view paper Downloaded 2 900 times Download rankings all-time: Site-wide: 1 885 out of 86 686 In immunology: 47 out of 2 468 Year to date: Site-wide: 319 out of 86 686 Since beginning of last month:
Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein bioRxiv - Immunology Pub Date : 2020-05-13 DOI: 10 1101/2020 05 12 091462 Seth J Zost Pavlo Gilchuk Rita E Chen James Brett Case Joseph X Reidy Andrew Trivette Rachel S Nargi Rachel E Sutton Naveenchandra Suryadevara Elaine C Chen Elad Binshtein Swathi
Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein Authors: Seth J Zost Pavlo Gilchuk Rita E Chen James Brett Case Joseph X Reidy Andrew Trivette Rachel S Nargi Rachel E Sutton Naveenchandra Suryadevara Elaine C Chen Elad Binshtein Swathi Shrihari Mario Ostrowski Helen Y Chu Jonathan E Didier Keith W MacRenaris Taylor
Macrophages are mononuclear phagocytes that constitute a first line of defense against pathogens While lethal to many microbes they are the primary host cells of Leishmania spp parasites the obligate intracellular pathogens that cause leishmaniasis We conducted transcriptomic profiling of two Leishmania species and the human macrophage over the course of intracellular infection by using
Swift Amplicon SARS-CoV-2 Panel 98% viral genome coverage for variant detection of SARS-CoV-2 1 tube of target-specific multiplex primer pool 341 amplicons amplicon avg size 150bp 2 hours workflow (cDNA to library) Swift RNA/Rapid RNA Library Kit Viral RNA sequencing Supports as low as 100pg of viral RNA Maximize mapping rates genes detected and transcript coverage Fast workflow of
Study the human microbiome and discover novel pathogens Explore Sequencing Methods and Uses Accessible Whole-Genome Sequencing Using capillary electrophoresis-based Sanger sequencing the Human Genome Project took over 10 years and cost nearly $3 billion Next-generation sequencing in contrast makes large-scale whole-genome sequencing (WGS) accessible and practical for the
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